Mouse Complement Component 4b ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Complement Component 4b in biofluid samples, such as serum, plasma and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . C4b present in samples reacts with an anti-Mouse C4b capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-C4b detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active C4b in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse C4b. Detection Target: Mouse Complement Component 4b Reactivity: Mouse Range: 0.625-40ng/mL Sensitivity: 0.245ng/mL Storage Conditions: Store all components at 4┬░C This Mouse C4b activity assay is for the quantitative determination of Complement Component 4b in samples including serum, plasma and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.